Tg(Fadd/EGFP)#Jizh
Transgene Detail

Summary

• Symbol: Tg(Fadd/EGFP)#Jizh
• Name: transgene insertion, Jianke Zhang
• MGI ID: MGI:4943162
• Synonyms: FADD:GFP, FADD:GFP^flox
• Transgene: Tg(Fadd/EGFP)#Jizh Location: unknown
• Alliance: Tg(Fadd/EGFP)#Jizh page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Fadd/EGFP)#Jizh expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Fadd (MGI:109324)

• Mutation details: EGFP was cloned into a 12.5 kb mouse Fadd minigene within exon 2 at the 3' end of the coding region. LoxP sites were also inserted upstream of exon 1 and downstream of exon 2. The final construct was injected into one-celled mouse embryos. Fifteen transgenic lines were produced. Western blot analysis detected the expected fusion protein in thymus, spleen, and lymph nodes. (J:113207)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:113207 Zhang Y, et al., Conditional Fas-associated death domain protein (FADD): GFP knockout mice reveal FADD is dispensable in thymic development but essential in peripheral T cell homeostasis. J Immunol. 2005 Sep 1;175(5):3033-44
• All: 6 reference(s)