Pcsk9^tm1.2Prat
Targeted Allele Detail

Summary

• Symbol: Pcsk9^tm1.2Prat
• Name: proprotein convertase subtilisin/kexin type 9; targeted mutation 1.2, Annik Prat
• MGI ID: MGI:4943496
• Gene: Pcsk9 Location: Chr4:106299531-106321522 bp, - strand Genetic Position: Chr4, 49.67 cM
• Alliance: Pcsk9^tm1.2Prat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169834
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The proximal promoter and exon 1 were flanked by loxP sites and an FRT flanked neomycin selection cassette was inserted just downstream of the loxP site in intron 1. The neomycin selection cassette was subsequently excised by flp recombinase expression after crossing to Gt(ROSA)26Sor mice and cre expression after crossing to Tg(CMV-cre)1Ipc mice resulted in excision of the proximal promoter and exon 1. (J:169834)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcsk9 Mutation: 46 strains or lines available

References

• Original: J:169834 Zaid A, et al., Proprotein convertase subtilisin/kexin type 9 (PCSK9): hepatocyte-specific low-density lipoprotein receptor degradation and critical role in mouse liver regeneration. Hepatology. 2008 Aug;48(2):646-54
• All: 11 reference(s)