Myt1^{tm1(EGFP/icre)Ldh}
Targeted Allele Detail

Summary

• Symbol: Myt1^{tm1(EGFP/icre)Ldh}
• Name: myelin transcription factor 1; targeted mutation 1, Lynn D Hudson
• MGI ID: MGI:4943749
• Gene: Myt1 Location: Chr2:181405125-181469590 bp, + strand Genetic Position: Chr2, 103.77 cM
• Alliance: Myt1^{tm1(EGFP/icre)Ldh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:169913
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Myt1^{tm1(EGFP/icre)Ldh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Myt1 (MGI:1100535)

• Mutation details: A targeting vector was designed to disrupt exon 2 with a fusion of EGFP and the modified Cre-recombinase, iCre , followed by a loxP site, and an frt-flanked neomycin (neo) resistance cassette. The vector also changed the initiation codon at the beginning of exon 2 to a GTG to ensure translation of the EGFP/iCre fusion protein. The neomycin cassette was removed by crossing mice with a transgenic strain expressing Tg(ACTFLPe)9205Dym leaving a single frt site and a loxP site downstream of the EGFP/iCre fusion. Northern blot confirmed the absence of mRNA. At E18, fluorescent cells are distributed throughout the spinal cord. (J:169913)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Myt1 (mouse) Summary of all recombinase alleles driven by Myt1. Comparative matrix view of recombinase activities Alliance mouse Myt1 gene page

Expression

In Mice Carrying this Mutation:

17 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myt1 Mutation: 57 strains or lines available

References

• Original: J:169913 Hudson LD, et al., A tool for examining the role of the zinc finger myelin transcription factor 1 (Myt1) in neural development: Myt1 knock-in mice. Transgenic Res. 2011 Aug;20(4):951-61
• All: 3 reference(s)