Hprt1^{tm5(Myh7-GFP,Myh6-CFP)Unc}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm5(Myh7-GFP,Myh6-CFP)Unc}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 5, University of North Carolina
• MGI ID: MGI:4943867
• Synonyms: aMHC-CFP
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm5(Myh7-GFP,Myh6-CFP)Unc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139125
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a 5.5 kb mouse Myh7 (bMHC) promoter driving a nuclear GFP, two copies of chicken beta globin HS4 insulators, a 5.6 kb mouse Myh6 (aMHC) promoter driving a cytoplasmic CFP and the human Hprt promoter (plus exons 1 and 2) upstream of mouse exon 3. CFP is expressed at low levels in the cells of day old mice, but at high levels in virtually all cells by postnatal day 10. GFP expression did not occur in adult cardiac myocytes. (J:139125)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:139125 Pandya K, et al., Discordant on/off switching of gene expression in myocytes during cardiac hypertrophy in vivo. Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):13063-8
• All: 1 reference(s)