Prag1^{tm1a(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Prag1^{tm1a(KOMP)Mbp}
• Name: PEAK1 related kinase activating pseudokinase 1; targeted mutation 1a, Mouse Biology Program, UC Davis
• MGI ID: MGI:4944311
• Synonyms: NACK^KOF
• Gene: Prag1 Location: Chr8:36562015-36614939 bp, + strand Genetic Position: Chr8, 22.78 cM
• Alliance: Prag1^{tm1a(KOMP)Mbp} page
• IMPC: Prag1 gene page

Mutation origin

• Mutant Cell Lines: DEPD0007_8_A06, DEPD0007_8_B04, DEPD0007_8_B06, DEPD0007_8_C04, DEPD0007_8_D01, DEPD0007_8_D02, DEPD0007_8_E03, DEPD0007_8_F04, DEPD0007_8_G05, DEPD0007_8_H01
• Germline Transmission: Earliest citation of germline transmission: J:214228
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 36569229 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 36571785. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064, J:214228)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Prag1 Mutation: 62 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 2 reference(s)