Nrxn3^tm4.1Sud
Targeted Allele Detail

Summary

• Symbol: Nrxn3^tm4.1Sud
• Name: neurexin III; targeted mutation 4.1, Thomas C Sudhof
• MGI ID: MGI:4946102
• Synonyms: Nrx3^ss4+, NS4
• Gene: Nrxn3 Location: Chr12:88689646-90301709 bp, + strand Genetic Position: Chr12, 42.94 cM
• Alliance: Nrxn3^tm4.1Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:170231
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector was designed to insert a loxP site upstream of exon 20 (alternative splice site 4) followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 20. The less common splice acceptor sequence of exon 20 (TGTTTTTTAAACTTTAAAG) was substituted with a much more common one (TGTTTTTTCGTCTTCCTAG) to make the exon always incorporated in the transcript. (J:170231)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Nrxn3^tm4.1Sud/Nrxn3^tm4.1Sud	involves: 129S1/Sv * 129X1/SvJ
cn2	Nrxn3^tm4.1Sud/Nrxn3^tm4.1Sud	involves: 129S1/Sv * 129X1/SvJ

Phenotypes:

Affected Systems	hm1	cn2
show or hide all annotated terms
nervous system	√	N
nervous system phenotype		N
abnormal synapse morphology	√
abnormal postsynaptic density morphology	√
reduced long-term potentiation	√
abnormal miniature excitatory postsynaptic currents	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nrxn3 Mutation: 80 strains or lines available

References

• Original: J:170231 Sudhof TC, Direct Data Submission 2011/04/11. MGI Direct Data Submission. 2011
• All: 3 reference(s)