Tg(BEST1-cre)1Jdun
Transgene Detail

Summary

• Symbol: Tg(BEST1-cre)1Jdun
• Name: transgene insertion 1, Joshua L Dunaief
• MGI ID: MGI:4946605
• Synonyms: BEST1-cre
• Transgene: Tg(BEST1-cre)1Jdun Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Tg(BEST1-cre)1Jdun page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutations: Insertion, Intergenic deletion
• Tg(BEST1-cre)1Jdun expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  BEST1 (7439)

  Best1

  (MGI:1346332)

• Mutation details: A fragment of the human BEST1 promoter (nucleotides -585 to +38) drives cre recombinase cDNA, the SV40 t-antigen intron, and HSV-TK polyA in the construct. This was purified and microinjected into C57BL/6 zygotes. Six founders were generated with one F2 line that showed the highest percentage of cre immunoreactivity in the RPE was selected for further study. Expression of cre was mosaic, with 50-90% of RPE cells being labeled. This line has a transgene copy number of 20 determined by qPCR analysis (J:170522) or 5 copies (J:240920) inserted about 58 kb upstream of Tmem108 (chr9:103,722,073; NCBI37/mm9) with a 120 bp deletion at the transgene integration site. (J:170522, J:240920)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	BEST1 (human) Summary of all recombinase alleles driven by BEST1. Comparative matrix view of recombinase activities Alliance human BEST1 gene page

Disease models

Expression

In Mice Carrying this Mutation:

3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:170522 Iacovelli J, et al., Generation of Cre Transgenic Mice with Postnatal RPE-Specific Ocular Expression. Invest Ophthalmol Vis Sci. 2011 Mar;52(3):1378-83
• All: 42 reference(s)