Tg(Hsd17b1-icre/ERT2)3Casa
Transgene Detail

Summary

• Symbol: Tg(Hsd17b1-icre/ERT2)3Casa
• Name: transgene insertion 3, Emilio Casanova
• MGI ID: MGI:4946612
• Synonyms: Hsd17b1-iCreER^T2
• Transgene: Tg(Hsd17b1-icre/ERT2)3Casa Location: unknown
• Alliance: Tg(Hsd17b1-icre/ERT2)3Casa page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Hsd17b1-icre/ERT2)3Casa expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Hsd17b1

• Mutation details: A 200kb BAC containing the Hsd17b1 gene was modified by homologous recombination to generate the construct. The open reading frame sequence for codon-improved cre recombinase (icre) fused to a mutated ligand-binding domain (LBD) of the human estrogen receptor (ERT2) was inserted into the exon containing the transcription initiation codon of the Hsd17b1 gene. The initial targeting vector also contained the bovine growth hormone polyA signal and an Frt-flanked ampicillin cassette. The amp marker was subsequently removed from targeted by clones upon electroporation of a plasmid expressing Flp recombinase. Purified BAC clones were injected into (C57BL/6 x CBA)F1 oocytes. Three lines expressing the transgene were generated. Lines 1 and 2 were shown to contain 2 copies of the insert by Southern blot, while line 3 had 8 copies. Line 3 mRNA levels of icre/ERT2 were highest in ovaries and this line was used for subsequent analyses. (J:170001)

Generation of Tg(Hsd17b1-icre/ERT2)3Casa

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hsd17b1 (species not specified) Summary of all recombinase alleles driven by Hsd17b1. Comparative matrix view of recombinase activities

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:170001 Grabner B, et al., A mouse tool for conditional mutagenesis in ovarian granulosa cells. Genesis. 2010 Oct 1;48(10):612-7
• All: 2 reference(s)