Tg(Adipoq-icre/ERT2)1Soff
Transgene Detail

Summary

• Symbol: Tg(Adipoq-icre/ERT2)1Soff
• Name: transgene insertion 1, Stefan Offermanns
• MGI ID: MGI:4946615
• Synonyms: Adipoq creER(T)², AdipoqCreER^T2
• Transgene: Tg(Adipoq-icre/ERT2)1Soff Location: unknown
• Alliance: Tg(Adipoq-icre/ERT2)1Soff page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Adipoq-icre/ERT2)1Soff expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Adipoq (MGI:106675)

• Mutation details: A targeting vector containing cDNA encoding icre fused to the mutated form of the ligand-binding domain of the human estrogen receptor followed by an Frt-flanked ampicillin resistance marker was generated. Homologous recombination resulted in the insertion of the vector into the start codon of the Adipoq locus present in a BAC clone (RP23-112M7; this BAC also contained the genes Kng1, Eif4a2, and Rfc4.) Flp-mediated excision removed the ampicillin marker. Five founders were obtained and crossed with lacZ reporter mice. Progeny of one founder displayed homogeneous lacZ expression in epididymal, mesenteric, and retroperitoneal white adipose tissue after tamoxifen treatment. (J:170002)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Adipoq (mouse) Summary of all recombinase alleles driven by Adipoq. Alliance mouse Adipoq gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:170002 Sassmann A, et al., Tamoxifen-inducible Cre-mediated recombination in adipocytes. Genesis. 2010 Oct 1;48(10):618-25
• All: 53 reference(s)