Tg(Kiss1-cre)J2-4Cfe
Transgene Detail

Summary

• Symbol: Tg(Kiss1-cre)J2-4Cfe
• Name: transgene insertion J2-4, Carol F Elias
• MGI ID: MGI:4946649
• Synonyms: J2-4, Kiss1-Cre
• Transgene: Tg(Kiss1-cre)J2-4Cfe Location: unknown
• Alliance: Tg(Kiss1-cre)J2-4Cfe page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Kiss1-cre)J2-4Cfe expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Kiss1 (MGI:2663985)

• Mutation details: A BAC containing the entire Kiss1 coding region with approximately 109 kb of upstream sequence and 69 kb of downstream sequence (RP24-299B2) was modified by ET-cloning recombineering methods. A DNA fragment encoding cre recombinase followed by the SV40 polyadenylation signal and an Frt-flanked kanamycin resistance marker was inserted at the translational start site of Kiss1. The resistance marker was deleted by arabinose induction of Flp recombinase in bacterial cells during cloning. The purified transgenic construct was injected into one-cell stage embryos from C57BL/6 mice. Three lines (J2-4, J2-4, and J2-6) were generated from the RP24-299B2 construct. J2-4 exhibited cre activity in known areas of Kiss1 mRNA expression and was analyzed in detail. (J:170260)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Kiss1 (mouse) Summary of all recombinase alleles driven by Kiss1. Comparative matrix view of recombinase activities Alliance mouse Kiss1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:170260 Cravo RM, et al., Characterization of Kiss1 neurons using transgenic mouse models. Neuroscience. 2011 Jan 26;173:37-56
• All: 36 reference(s)