Gt(ROSA)26Sor^{tm34.1(CAG-Syp/tdTomato)Hze}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm34.1(CAG-Syp/tdTomato)Hze}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 34.1, Hongkui Zeng
• MGI ID: MGI:4947243
• Synonyms: Ai34, R26^{synaptophysin-tdTomato}, Rosa-CAG-LSL-Syp-tdTomato-deltaNeo
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm34.1(CAG-Syp/tdTomato)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:170755
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The Rosa-CAG-LSL-Synaptophysin-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), a Synaptophysin-tdTomato fusion protein sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. To create the Synaptophysin-tdTomato fusion protein sequence, a mouse synaptophysin cDNA sequence (encoding a 308 amino acid transcript with all but the first six amino acids of the full-length protein) was fused in-frame via a six nucleotide linker to the amino terminus of the tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)). PhiC31-mediated recombination removed the neo cassette. (J:170755)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:170755 Zeng H, Direct Data Submission 2011/04/15. MGI Direct Data Submission. 2011
• All: 64 reference(s)