Et(icre)23033Rdav
Transgene Detail

Summary

• Symbol: Et(icre)23033Rdav
• Name: enhancer trap 23033, Ron Davis
• MGI ID: MGI:4947561
• Transgene: Et(icre)23033Rdav Location: unknown
• Alliance: Et(icre)23033Rdav page

Transgene origin

• Strain of Origin: C57BL/6J
• Project Collection: Neuroscience Blueprint cre

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Et(icre)23033Rdav expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Et(icre)23033Rdav (MGI:4947600)

• Mutation details: An enhancer trap transgenic approach was used to generate these mice. The Calb1-icre transgene was designed with a 300 bp minimal promoter from the mouse calbindin 1 locus, a codon-improved cre recombinase (icre; improved with mammalian codon usage, no putative cryptic splice sites, altered stop codon, and reduced CpG content to limit the chances of epigenetic silencing in mammals), and an SV40 late polyA signal. This transgene was injected into C57BL/6J mouse embryos. Founders were crossed to C57BL/6 animals. Animals were crossed to B6-Gt(ROSA)26Sor^tm1Sor mice to assess cre activity/transmission. Cre positive, lacZ negative mice were subsequently bred to C57BL/6 mice to create the 23033 line. (J:150856)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Et(icre)23033Rdav (mouse) Summary of all recombinase alleles driven by Et(icre)23033Rdav. Alliance mouse Et(icre)23033Rdav gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:150856 Davis R, Large-scale enhancer trap project to generate transgenic cre alleles by Ron Davis at Baylor University. MGI Direct Data Submission. 2009
• All: 1 reference(s)