Orc3^tm1.1Zhua
Targeted Allele Detail

Summary

• Symbol: Orc3^tm1.1Zhua
• Name: origin recognition complex, subunit 3; targeted mutation 1.1, Zhen Huang
• MGI ID: MGI:4947979
• Gene: Orc3 Location: Chr4:34570796-34614944 bp, - strand Genetic Position: Chr4, 16.81 cM, cytoband A3
• Alliance: Orc3^tm1.1Zhua page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:170581
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An FRT flanked neo cassette with a 5' loxP site was inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 7. Flp-mediated recombination removed the neo cassette leaving exons 5 through 7 floxed. An 84% reduction in protein expression was confirmed by western blot analysis on E13.5 brain extracts (J:170581)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Orc3^tm1.1Zhua/Orc3^tm1.1Zhua Emx1^tm1(cre)Krj/Emx1^+	involves: 129S2/SvPas

Phenotypes:

Affected Systems	cn1
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cellular	√
abnormal radial glial cell morphology	√
decreased radial glial cell number	√
decreased neuronal precursor proliferation	√
nervous system	√
nervous system phenotype	N
abnormal radial glial cell morphology	√
decreased radial glial cell number	√
decreased neuronal precursor proliferation	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Orc3 Mutation: 48 strains or lines available

References

• Original: J:170581 Kwon HJ, et al., Radial glia regulate Cajal-Retzius cell positioning in the early embryonic cerebral cortex. Dev Biol. 2011 Mar 1;351(1):25-34
• All: 1 reference(s)