Trim28^tm1Fca
Targeted Allele Detail

Summary

• Symbol: Trim28^tm1Fca
• Name: tripartite motif-containing 28; targeted mutation 1, Florence Cammas
• MGI ID: MGI:4948085
• Synonyms: Kap1^V488A-L490A, TIF1Beta^HP1box, TIF1Beta^L2HP1box
• Gene: Trim28 Location: Chr7:12758079-12764959 bp, + strand Genetic Position: Chr7, 7.73 cM, cytoband A2
• Alliance: Trim28^tm1Fca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:170392
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site flanked hygromycin resistance gene cassette and a mutated version of exon 12 containing two codon substitutions, p.V488A and p.L490A, were inserted via homologous recombination. The mutations in exon 12 disrupt the interaction between this protein and Cbx1, Cbx5, and Cbx3. Confocal microscopic analysis indicates that the association of the protein with the heterochromatin is disrupted in homozygous embryos at E8.5. (J:170392, J:171108)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trim28 Mutation: 36 strains or lines available

References

• Original: J:170392 Herzog M, et al., TIF1beta association with HP1 is essential for post-gastrulation development, but not for Sertoli cell functions during spermatogenesis. Dev Biol. 2011 Feb 15;350(2):548-58
• All: 5 reference(s)