Hcrtr2^tm1Tesc
Targeted Allele Detail

Summary

• Symbol: Hcrtr2^tm1Tesc
• Name: hypocretin (orexin) receptor 2; targeted mutation 1, Thomas E Scammell
• MGI ID: MGI:4949132
• Synonyms: OX2R TD
• Gene: Hcrtr2 Location: Chr9:76133162-76230905 bp, - strand Genetic Position: Chr9, 42.48 cM
• Alliance: Hcrtr2^tm1Tesc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:170824
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed transcription disrupter cassette was inserted into intron 1, 137 bp upstream of exon 2 via homologous recombination. The transcription disrupter cassette includes an engrailed 2 splice acceptor, SV40 enhancer, Neo, and a Myc-associated zinc finger protein-binding site. RT-PCR analysis confirmed the absence of full length transcript and the presence of a mutant mRNA. (J:170824)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hcrtr2 Mutation: 38 strains or lines available

References

• Original: J:170824 Mochizuki T, et al., Orexin receptor 2 expression in the posterior hypothalamus rescues sleepiness in narcoleptic mice. Proc Natl Acad Sci U S A. 2011 Mar 15;108(11):4471-6
• All: 2 reference(s)