Tg(Col1a1-cre/ERT2)1Crm
Transgene Detail

Summary

• Symbol: Tg(Col1a1-cre/ERT2)1Crm
• Name: transgene insertion 1, Benoit de Crombrugghe
• MGI ID: MGI:4949435
• Synonyms: Col1a1-CreERT2
• Transgene: Tg(Col1a1-cre/ERT2)1Crm Location: unknown
• Alliance: Tg(Col1a1-cre/ERT2)1Crm page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Col1a1-cre/ERT2)1Crm expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Col1a1 (MGI:88467)

• Mutation details: The transgene consists of a tamoxifen-inducible cre recombinase driven by the 2.3-kb mouse Col1a1, collagen, type I, alpha 1, promoter. The transgene insert contains a fusion product involving cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Inducible cre recombinase activity is detected in the osteoblasts of the long bones of the limbs and in the ribs, vertebrae and calvaria in 18.5 embryonic day aged and 18 day old mice. Inducible cre recombinase activity was not detected in chondrocytes. (J:94972)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Col1a1 (mouse) Summary of all recombinase alleles driven by Col1a1. Comparative matrix view of recombinase activities Alliance mouse Col1a1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:94972 Kim JE, et al., Transgenic mice expressing a ligand-inducible cre recombinase in osteoblasts and odontoblasts: a new tool to examine physiology and disease of postnatal bone and tooth. Am J Pathol. 2004 Dec;165(6):1875-82
• All: 31 reference(s)