Kcnu1^tm1.1Clin
Targeted Allele Detail

Summary

• Symbol: Kcnu1^tm1.1Clin
• Name: potassium channel, subfamily U, member 1; targeted mutation 1.1, Christopher J Lingle
• MGI ID: MGI:4949762
• Synonyms: Slo3-eGFP⁺
• Gene: Kcnu1 Location: Chr8:26339651-26427967 bp, + strand Genetic Position: Chr8, 14.17 cM, cytoband A3
• Alliance: Kcnu1^tm1.1Clin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171225
• Parent Cell Line: B6/BLU (ES Cell)
• Strain of Origin: C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 27. A cassette (5' to 3': EGFP, loxP, FRT, neo resistance marker, loxP, and FRT) was inserted downstream of exon 27. Flp-mediated recombination removed the neo cassette. Exon 27 and the EGFP remain floxed. (J:171225)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kcnu1 Mutation: 63 strains or lines available

Notes

The primary investigator indicates that the ES cell line used was C57BL/6-derived BLU.

References

• Original: J:171225 Zeng XH, et al., Deletion of the Slo3 gene abolishes alkalization-activated K+ current in mouse spermatozoa. Proc Natl Acad Sci U S A. 2011 Apr 5;108(14):5879-84
• All: 4 reference(s)