Gnas/Nespastm1.1Jop
Targeted Allele Detail
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Symbol: |
Gnas/Nespastm1.1Jop |
Name: |
GNAS complex locus; targeted mutation 1.1, Jo Peters |
MGI ID: |
MGI:4950063 |
Synonyms: |
Nesp-Tint2 |
Gene: |
Gnas Location: Chr2:174126113-174188537 bp, + strand Genetic Position: Chr2, 97.89 cM, cytoband E1-H3
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Alliance: |
Gnas/Nespastm1.1Jop page
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Allele Type: |
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Targeted (Hypomorph) |
Mutation: |
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Insertion
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Mutation details: A cassette containing a reverse orientation (relative to Nespas) polyadenylation sequence and a self-excising neo cassette was inserted into exon 1. Cre-mediated recombination removed the neo cassette. The 94% reduction in Nespas transcript expression was confirmed by RT-qPCR on newborn brain extracts with paternal inheritance of the allele. RT-qPCR on newborn brain extracts confirmed reduced Nesp (Gnas) expression when the allele is inherited marternally but increased expression when the allele is paternally inherited.
(J:171195)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gnas Mutation: |
54 strains or lines available
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Original: |
J:171195 Williamson CM, et al., Uncoupling antisense-mediated silencing and DNA methylation in the imprinted gnas cluster. PLoS Genet. 2011 Mar;7(3):e1001347 |
All: |
2 reference(s) |
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