Cspg4^tm1(cre)Akik
Targeted Allele Detail

Summary

• Symbol: Cspg4^tm1(cre)Akik
• Name: chondroitin sulfate proteoglycan 4; targeted mutation 1, Akiko Nishiyama
• MGI ID: MGI:4950067
• Synonyms: NG2CreKI, NG2 KO
• Gene: Cspg4 Location: Chr9:56772388-56807154 bp, + strand Genetic Position: Chr9, 30.32 cM
• Alliance: Cspg4^tm1(cre)Akik page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171287
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutations: Insertion, Intragenic deletion
• Cspg4^tm1(cre)Akik expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cspg4 (MGI:2153093)

• Mutation details: A targeting vector was designed so that it contained a homology arm-flanked nlsCre cDNA (nls;nuclear localization signal), a pgk-neo cassette 3' of the nlscre, and an MC1-driven diphtheria toxin selection gene. After homologous recombination in RENKA ES cells, targeted clones had the cre cDNA inserted immediately after the translational initiation codon of Cspg4. ES cell clones were injected into 8-cell CD1 embryos to produce germline chimeras to establish the knock-in line. (J:171287)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cspg4 (mouse) Summary of all recombinase alleles driven by Cspg4. Alliance mouse Cspg4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cspg4 Mutation: 101 strains or lines available

References

• Original: J:171287 Hill RA, et al., NG2 cells are uniformly distributed and NG2 is not required for barrel formation in the somatosensory cortex. Mol Cell Neurosci. 2011 Apr;46(4):689-98
• All: 2 reference(s)