Col1a1^{tm2(tetO-GFP/RNAi:Trp53)Slowe}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm2(tetO-GFP/RNAi:Trp53)Slowe}
• Name: collagen, type I, alpha 1; targeted mutation 2, Scott Lowe
• MGI ID: MGI:4950577
• Synonyms: tet-shp53, TG-p53.1224
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm2(tetO-GFP/RNAi:Trp53)Slowe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171191
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm2(tetO-GFP/RNAi:Trp53)Slowe} involves 1 genes/genome features (Trp53) View all
• Mutation details: RMCE on KH2 cells inserted a cassette that contains the tetracycline promoter and a GFP fusion with an Mir30e-based short hairpin sequence targeting Trp53 (p53.1224) into the 3'UTR. (J:171191)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:171191 Premsrirut PK, et al., A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell. 2011 Apr 1;145(1):145-58
• All: 2 reference(s)