Dazap1^tm1Pyen
Targeted Allele Detail

Summary

• Symbol: Dazap1^tm1Pyen
• Name: DAZ associated protein 1; targeted mutation 1, Pauline Yen
• MGI ID: MGI:4950677
• Synonyms: Dazap1^fn, Dazap^tm115FnYen
• Gene: Dazap1 Location: Chr10:80097320-80124242 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Dazap1^tm1Pyen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171493
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: One loxP site was inserted just upstrean of exon 2 and a floxed neomycin selection cassette in reverse orientation was inserted in intron 4. Exons 2-4 which encode the first RNA binding domain were thus flanked by loxP sites. This allele expresses an aberrant transcript 500 bp longer than expected because of an additional exon encoding an additional 166 amino acids. Low amounts of a 64 kDalton protein are produced. (J:171493)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dazap1 Mutation: 40 strains or lines available

References

• Original: J:171493 Hsu LC, et al., DAZAP1, an hnRNP protein, is required for normal growth and spermatogenesis in mice. RNA. 2008 Sep;14(9):1814-22
• All: 2 reference(s)