Gt(ROSA)26Sor^{tm38.1(CAG-GCaMP3)Hze}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm38.1(CAG-GCaMP3)Hze}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 38.1, Hongkui Zeng
• MGI ID: MGI:4950879
• Synonyms: Ai38delta, Ai38deltaneo, Ai38D (Rosa-CAG-LSL-GCaMP3-WPRE), Gt(ROSA)26Sor^{tm38.1(CAG-MYLK/EGFP*/Calm*)Hze}, Rosa-CAG-LSL-GCaMP3-deltaNeo, Rosa-CAG-LSL-GCaMP3-WPRE
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm38.1(CAG-GCaMP3)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171735
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The Rosa-CAG-LSL-GCaMP3-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), a GCaMP3 fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. The GCaMP3 fusion gene contains a cDNA sequence encoding the chicken smooth muscle M13 fragment of myosin light chain kinase, a circularly permutated EGFP (amino acid residues 149-238 followed by amino acid residues 1-144 (including M153K, V163A, S175G, D180Y, T203V, A206K, and V93I mutations to increase dynamic range and baseline fluorescence), and a DNA fragment encoding amino acid residues 2-148 of the rat calmodulin (modified to harbor the N60D mutation to increase the fluorescence change for small calcium transients). PhiC31-mediated recombination removed the neo cassette. (J:171735)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:171735 Feng H, Direct Data Submission 2011/05/19. MGI Direct Data Submission. 2011
• All: 2 reference(s)