Cryba1^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Cryba1^{tm1a(EUCOMM)Hmgu}
• Name: crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4951042
• Synonyms: beta3-, betaA3 KO
• Gene: Cryba1 Location: Chr11:77609441-77616109 bp, - strand Genetic Position: Chr11, 46.74 cM, cytoband B-C1
• Alliance: Cryba1^{tm1a(EUCOMM)Hmgu} page
• IMPC: Cryba1 gene page

Nuclear cataracts in Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} mice

Show the 8 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: HEPD0727_4_A03, HEPD0727_4_B04, HEPD0727_4_C03, HEPD0727_4_C04, HEPD0727_4_D04, HEPD0727_4_E01, HEPD0727_4_F03, HEPD0727_4_F04, HEPD0727_4_G02, HEPD0727_4_G03, HEPD0727_4_H01, HEPD0727_4_H02, HEPD0727_4_H03
• Germline Transmission: Earliest citation of germline transmission: J:235696
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 77611369 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 77612125. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Generation of the Cryba1^tm1a(EUCOMM)Hmgu allele

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Cryba1 Mutation: 11 strains or lines available

Notes

Special note about the mutation generated in J:235696: qRT-PCR analysis confirmed the absence of mRNA transcripts in homozygous mutant lenses. Western blot analysis using a polyclonal anti-betaA3-crystallin antibody revealed that reactivity corresponding to a dimer (50 kDa) is completely absent in homozygous lenses and reduced to 50% in heterozygous lenses. However, a band of severely reduced intensity is still detectable in homozygous lenses at the 25 kDa position. Similar results are observed using a monoclonal antibody, suggesting that a small amount of protein is still produced in homozygous lenses.

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)