Fam161a^{Gt(462E7)Cmhd}
Gene trapped Allele Detail

Summary

• Symbol: Fam161a^{Gt(462E7)Cmhd}
• Name: family with sequence similarity 161, member A; gene trap 462E7, Centre for Modeling Human Disease
• MGI ID: MGI:4955130
• Gene: Fam161a Location: Chr11:22957531-22980788 bp, + strand Genetic Position: Chr11, 14.25 cM, cytoband A3.3
• Alliance: Fam161a^{Gt(462E7)Cmhd} page

Mutation origin

• Mutant Cell Line: 462E7
• Germline Transmission: Earliest citation of germline transmission: J:214319
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: UPA
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. The UPA-vector gene trap containing GFP and a neomycin resistance gene inserted into exon 3. This insertion disrupts the highly conserved UPF0564 domain after amino acid 362. Western blot confirmed absence of the full-length protein in the retina and immunohistochemistry of retinal sections shows weak expression of a truncated protein. (J:214319)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  CMHD-GT_462E7-5S	FI279721 ; (MGI Seq Detail)	5'Splinkerette PCR	Chr11:22970406-22970670 bp (+)
  CMHD-GT_462E7-3	ET201387 ; (MGI Seq Detail)	3' RACE	Chr11:22970693-22971514 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  1 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fam161a Mutation: 32 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)