Chat/Slc18a3^tm1Vpra
Targeted Allele Detail

Summary

• Symbol: Chat/Slc18a3^tm1Vpra
• Name: choline O-acetyltransferase; targeted mutation 1, Vania Prado
• MGI ID: MGI:4999561
• Synonyms: VAChT^FloxNeo
• Gene: Chat Location: Chr14:32130160-32187866 bp, - strand Genetic Position: Chr14, 19.4 cM
• Alliance: Chat/Slc18a3^tm1Vpra page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:153908
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: One loxP sequence was added 260 bp upstream from the translational initiation codon of Slc18a3 (within the first intron of Chat), and a second loxP was added approximately 1.5 kb downstream from the stop codon. This flanking region included the second exon of Chat. The TK-Neo cassette was added immediately after the second loxP and was followed by a third loxP (all in the same direction). QPCR confirmed reduced transcript expression in the brain and spinal cord. (J:153908)

Generation of the Chat/Slc18a3^tm1Vpra, Chat/Slc18a3^tm1.1Vpra and Chat/Slc18a3^tm1.2Vpra alleles

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chat Mutation: 58 strains or lines available

References

• Original: J:153908 de Castro BM, et al., The vesicular acetylcholine transporter is required for neuromuscular development and function. Mol Cell Biol. 2009 Oct;29(19):5238-50
• All: 4 reference(s)