Col1a1^{tm10(tetO*-RFP/RNAi:Rpa3)Slowe}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm10(tetO*-RFP/RNAi:Rpa3)Slowe}
• Name: collagen, type I, alpha 1; targeted mutation 10, Scott Lowe
• MGI ID: MGI:4999905
• Synonyms: Col1a1^{tm1(TREtight-turboRFP-shRpa3.455)Swl}, TtR-shRpa3.455
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm10(tetO*-RFP/RNAi:Rpa3)Slowe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171348
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{tm10(tetO*-RFP/RNAi:Rpa3)Slowe} involves 1 genes/genome features (Rpa3) View all
• Mutation details: RMCE on KH2 cells inserted a cassette that contains a modified tetracycline promoter (TREtight or Tt; expression in the absence of doxycycline is 40-fold less than with the standard tetO promoter) and a fast-maturing RFP (turboRFP; tRFP) fusion with an Mir30e-based short hairpin sequence targeting Rpa3 (shRpa3.455) into the 3'UTR. (J:171348)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:171348 McJunkin K, et al., Reversible suppression of an essential gene in adult mice using transgenic RNA interference. Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7113-8
• All: 1 reference(s)