Gpr55^tm1Tigm
Targeted Allele Detail

Summary

• Symbol: Gpr55^tm1Tigm
• Name: G protein-coupled receptor 55; targeted mutation 1, Texas A&M Institute for Genomic Medicine
• MGI ID: MGI:5000016
• Synonyms: GPR55^-
• Gene: Gpr55 Location: Chr1:85866039-85888729 bp, - strand Genetic Position: Chr1, 43.94 cM
• Alliance: Gpr55^tm1Tigm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171771
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S5/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was constructed to replace part of exon 2 of the Gpr55 gene (containing the entire coding region) with a selection cassette. Homologous recombination was carried out in 129SvEvBrd-derived ES cells, followed by injection of targeted ES cells into blastocyts. Loss of Gpr55 mRNA was confirmed by RT-PCR which demonsrated lack of Gpr55 cDNAs in homozygous animals. (J:171771)

Expression

In Mice Carrying this Mutation:

4 assay results

Tumor Data

List all tumor models in MMHCdb carrying Gpr55^tm1Tigm

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpr55 Mutation: 20 strains or lines available

References

• Original: J:171771 Wu CS, et al., Requirement of cannabinoid CB(1) receptors in cortical pyramidal neurons for appropriate development of corticothalamic and thalamocortical projections. Eur J Neurosci. 2010 Sep;32(5):693-706
• All: 16 reference(s)