Rbck1^tm1Kiwa
Targeted Allele Detail

Summary

• Symbol: Rbck1^tm1Kiwa
• Name: RanBP-type and C3HC4-type zinc finger containing 1; targeted mutation 1, Kazuhiro Iwai
• MGI ID: MGI:5000222
• Synonyms: HOIL-1L^-
• Gene: Rbck1 Location: Chr2:152158254-152174573 bp, - strand Genetic Position: Chr2, 74.83 cM
• Alliance: Rbck1^tm1Kiwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:158069
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 7 and 8 were replaced with a neomycin selection cassette. The absence of protein expression was confirmed by western blot analysis on liver extracts using antibody to the C-terminal part of the protein. Subsequent studies showed that an N-terminal portion of the protein containing the UBL domain was still present, possibly the product of a splice variant of the gene not affected by the mutation, or because of incomplete nonsense-mediated mRNA decay (NMD). (J:158069, J:334038)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rbck1 Mutation: 43 strains or lines available

References

• Original: J:158069 Tokunaga F, et al., Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation. Nat Cell Biol. 2009 Feb;11(2):123-32
• All: 8 reference(s)