Gabra2^tm1.1Geh
Targeted Allele Detail

Summary

• Symbol: Gabra2^tm1.1Geh
• Name: gamma-aminobutyric acid type A receptor subunit alpha 2; targeted mutation 1.1, Gregg E Homanics
• MGI ID: MGI:5000434
• Synonyms: Gabra2^HA
• Gene: Gabra2 Location: Chr5:71115735-71253192 bp, - strand Genetic Position: Chr5, 37.59 cM
• Alliance: Gabra2^tm1.1Geh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171967
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector was designed (by site-directed mutagenesis) to substitute a serine to histidine at amino acid position 270 and leucine to alanine at amino acid position 277 of exon 9. An FRT-flanked PGKneo cassette was inserted downstream of exon 9. Flp-mediated recombination removed the neo cassette and left a single FRT site downstream of exon 9. (J:171967)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabra2 Mutation: 36 strains or lines available

References

• Original: J:171967 Werner DF, et al., Inhaled anesthetic responses of recombinant receptors and knockin mice harboring alpha2(S270H/L277A) GABA(A) receptor subunits that are resistant to isoflurane. J Pharmacol Exp Ther. 2011 Jan;336(1):134-44
• All: 2 reference(s)