Oprm1^tm1Shlz
Targeted Allele Detail

Summary

• Symbol: Oprm1^tm1Shlz
• Name: opioid receptor, mu 1; targeted mutation 1, Stefan Schulz
• MGI ID: MGI:5000465
• Synonyms: MOR^S375A
• Gene: Oprm1 Location: Chr10:6708593-6988209 bp, + strand Genetic Position: Chr10, 1.85 cM, cytoband A2
• Alliance: Oprm1^tm1Shlz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:176122
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The coding sequence for serine at codon 375 (TCC) has been replaced using PCR with the coding sequence of alanine (GCC). An FRT flanked PKG-neomycin selection cassette was placed in intron 3. Exons 2 and 3 were also flanked by loxP sites. The selection cassette was subsequently removed by flp-mediated recombination. (J:176122)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Oprm1 Mutation: 54 strains or lines available

References

• Original: J:176122 Grecksch G, et al., Analgesic Tolerance to High-Efficacy Agonists But Not to Morphine Is Diminished in Phosphorylation-Deficient S375A {micro}-Opioid Receptor Knock-In Mice. J Neurosci. 2011 Sep 28;31(39):13890-6
• All: 3 reference(s)