Col5a3^tm1Dgr
Targeted Allele Detail

Summary

• Symbol: Col5a3^tm1Dgr
• Name: collagen, type V, alpha 3; targeted mutation 1, Daniel S Greenspan
• MGI ID: MGI:5000519
• Gene: Col5a3 Location: Chr9:20681353-20726363 bp, - strand Genetic Position: Chr9, 7.63 cM
• Alliance: Col5a3^tm1Dgr page

Reduced thickness of the adipocyte-rich hypodermal layer of Col5a3^tm1Dgr/Col5a3^tm1Dgr females

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171817
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A 1.2 kb genomic fragment including 3 C-propeptide exons was replaced with a neo cassette via homologous recombination. Immunoblot and RT-PCR analysis of E15.5 homozygous embryos confirmed the absence of RNA and protein expression. (J:171817)

Generation of the Col5a3^tm1Dgr allele

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col5a3 Mutation: 65 strains or lines available

References

• Original: J:171817 Huang G, et al., alpha3(V) collagen is critical for glucose homeostasis in mice due to effects in pancreatic islets and peripheral tissues. J Clin Invest. 2011 Feb 1;121(2):769-83
• All: 2 reference(s)