Tg(Phox2b-cre)3Jke
Transgene Detail

Summary

• Symbol: Tg(Phox2b-cre)3Jke
• Name: transgene insertion 3, Joel K Elmquist
• MGI ID: MGI:5005058
• Transgene: Tg(Phox2b-cre)3Jke Location: unknown
• Alliance: Tg(Phox2b-cre)3Jke page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Phox2b-cre)3Jke expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Phox2b (MGI:1100882)

• Mutation details: The mouse BAC RP24-95M11, containing the entire Phox2b locus and 130kb sequence upstream of the Phox2b locus (including part of the Limch1 locus) and 70kb of sequence downstream of the Phox2b locus was modified to produce the construct. A Cre recombinase sequence fused with an frt-flanked neomycin gene was inserted into the ATG start site of the Phox2b locus via homologous recombination/BAC recombineering. The frt-flanked neo was removed via transient transfection of an FLP-expressing vector during BAC recombineering. The resulting ~200 kb Phox2b-Cre BAC transgene was injected into C57BL/6J mouse pronuclei to produce independent founder lines. Founder line 3 was found with the greatest degree of nucleus of the solitary tract (NTS) cells expressing Cre recombinase. (J:172264)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Phox2b (mouse) Summary of all recombinase alleles driven by Phox2b. Comparative matrix view of recombinase activities Alliance mouse Phox2b gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:172264 Scott MM, et al., Leptin receptor expression in hindbrain Glp-1 neurons regulates food intake and energy balance in mice. J Clin Invest. 2011 Jun 1;121(6):2413-21
• All: 42 reference(s)