Tg(IghmHyHEL10,IgkHyHEL10)4Ccg
Transgene Detail

Summary

• Symbol: Tg(IghmHyHEL10,IgkHyHEL10)4Ccg
• Name: transgene insertion 4, Christopher C Goodnow
• MGI ID: MGI:5006835
• Synonyms: Ceres, IgM-only anti-HEL transgene, MM-4, MM4, Tg(IghelM)Ccg, Tg(IghmMM)4Rbr
• Transgene: Tg(IghmHyHEL10,IgkHyHEL10)4Ccg Location: unknown
• Alliance: Tg(IghmHyHEL10,IgkHyHEL10)4Ccg page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(IghmHyHEL10,IgkHyHEL10)4Ccg expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ighm (MGI:96448)
  mouse	Igk (MGI:96494)

• Mutation details: Fertilized mouse eggs were coinjected with mouse immunoglobulin heavy and light chain transgene constructs whose rearranged heavy chain VDJ segments and kappa light chain VJ segments were derived from the HyHEL10 plasmacytoma cell line, which produces a high affinity antibody to chicken lysozyme (commonly called hen egg lysozyme (HEL)). The heavy chain variable segments are joined to the Ighm switch region followed by the Ighm constant region and membrane terminal exons of the Igh^a allotype, and the light chain variable segments to the Igk constant region and 3' flanking DNA of the Igk^a allotype. Each construct is expressed from the promoter upstream of the leader sequence of the V-region segment. Line 4 was estimated by Southern blot analysis to carry 4-6 and 2-3 copies of the Ighm and Igk constructs, respectively. (J:78308, J:139863)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

This is one of two lines characterized; the other was line 7. (J:139863)

References

• Original: J:78307 Hartley SB, et al., Elimination from peripheral lymphoid tissues of self-reactive B lymphocytes recognizing membrane-bound antigens. Nature. 1991 Oct 24;353(6346):765-9
• All: 5 reference(s)