Pak2^tm1Rjak
Targeted Allele Detail

Summary

• Symbol: Pak2^tm1Rjak
• Name: p21 (RAC1) activated kinase 2; targeted mutation 1, Rolf Jakobi
• MGI ID: MGI:5006977
• Synonyms: PAK-2D212N-Neo+
• Gene: Pak2 Location: Chr16:31835108-31898160 bp, - strand Genetic Position: Chr16, 22.4 cM
• Alliance: Pak2^tm1Rjak page

Pak2^tm1Rjak/Pak2^tm1Rjak embryos are smaller and less developed

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171914
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Nucleotide substitutions Mutation details: Nucleotide substitutions in exon 7 result in the amino acid substitution of asparagine for aspartic acid at position 212 (D212N). A floxed neo cassette was inserted downstream of the modified exon 7. The absence of protein expression was confirmed by western blot analysis. (J:171914)

Generation of the Pak2^tm1Rjak allele

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pak2 Mutation: 66 strains or lines available

References

• Original: J:171914 Marlin JW, et al., Functional PAK-2 knockout and replacement with a caspase cleavage-deficient mutant in mice reveals differential requirements of full-length PAK-2 and caspase-activated PAK-2p34. Mamm Genome. 2011 Jun;22(5-6):306-17
• All: 1 reference(s)