Gt(ROSA)26Sor^{tm2(NOTCH3*C455R)Sat}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(NOTCH3*C455R)Sat}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Spyros Artavanis-Tsakonas
• MGI ID: MGI:5007800
• Synonyms: NOTCH3^C455R
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(NOTCH3*C455R)Sat} page

Vascular smooth muscle cell abnormalities in Gt(ROSA)26Sor^{tm3(NOTCH3*R1031C)Sat}/Gt(ROSA)26Sor⁺ Tg(Tagln-cre)1Her/0 and Gt(ROSA)26Sor^{tm2(NOTCH3*C455R)Sat}/Gt(ROSA)26Sor⁺ Notch3^{Gt(PST033)Byg}/Notch3^{Gt(PST033)Byg} Tg(Tagln-cre)1Her/0 mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171887
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(NOTCH3*C455R)Sat} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  NOTCH3 (4854)

  Notch3

  (MGI:99460)

• Mutation details: A targeting vector containing a splice acceptor site, a floxed PGK neomycin cassette, a human NOTCH3 cDNA with a C455R mutation and an IRES-nuclear GFP sequence was inserted via homologous recombination. (J:171887)

Schematic of the Gt(ROSA)26Sor^{tm1(NOTCH3)Sat}, Gt(ROSA)26Sor^{tm2(NOTCH3*C455R)Sat} and Gt(ROSA)26Sor^{tm3(NOTCH3*R1031C)Sat} constructs

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:171887 Arboleda-Velasquez JF, et al., PNAS Plus: Hypomorphic Notch 3 alleles link Notch signaling to ischemic cerebral small-vessel disease. Proc Natl Acad Sci U S A. 2011 May 24;108(21):E128-35
• All: 3 reference(s)