Gt(ROSA)26Sor^{tm35.1(CAG-aop3/GFP)Hze}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm35.1(CAG-aop3/GFP)Hze}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 35.1, Hongkui Zeng
• MGI ID: MGI:5008454
• Synonyms: Ai35, Ai35D (Rosa-CAG-LSL-Arch-GFP-WPRE), Arch-ER2, Rosa-CAG-LSL-ArchSSER2-EGFP-deltaNeo, ss-Arch-EGFP-ER2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm35.1(CAG-aop3/GFP)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:172633
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The Rosa-CAG-LSL-Arch-GFP-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), an Arch-GFP fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. The Arch-GFP fusion gene is flanked with small domain modifications designed to facilitate correct processing and localization of newly synthesized Arch-GFP protein. Specifically, this ss-Arch-GFP-ER2 construct was designed with the signal sequence (ss) from truncated MHC class I antigen (amino acids MVPCTLLLLLAAALAPTQTRA), a mammalian codon-optimized cDNA sequence encoding the Archaerhodopsin-3 protein (Arch, aR-3, or aop3 ; derived from the halophilic bacterium Halorubrum sodomense) fused in-frame via a 15 nucleotide linker to the amino terminus of a green fluorescent protein (GFP), and an endoplasmic reticulum exporting sequence (ER2) from the potassium channel Kir2.1 gene (amino acids FCYENEV) at the C-terminal of the GFP. PhiC31-mediated recombination replaced the neo cassette with the recombined attB/attP site (attL). (J:172633, J:182204)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:172633 Zeng H, Direct Data Submission 2011/06/21. MGI Direct Data Submission. 2011
• All: 58 reference(s)