Ascc2^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ascc2^{tm1a(EUCOMM)Wtsi}
• Name: activating signal cointegrator 1 complex subunit 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:5008937
• Gene: Ascc2 Location: Chr11:4587747-4635699 bp, + strand Genetic Position: Chr11, 2.94 cM
• Alliance: Ascc2^{tm1a(EUCOMM)Wtsi} page
• IMPC: Ascc2 gene page

Mutation origin

• Mutant Cell Lines: EPD0792_5_A05, EPD0792_5_A06, EPD0792_5_A08, EPD0792_5_B05, EPD0792_5_B07, EPD0792_5_C05, EPD0792_5_C06, EPD0792_5_C08, EPD0792_5_D07, EPD0792_5_D08, EPD0792_5_E06, EPD0792_5_F05, EPD0792_5_F07, EPD0792_5_F08, EPD0792_5_G05, EPD0792_5_G08, EPD0792_5_H05
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 4599308 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 4600097. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Ascc2 Mutation: 41 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 7 reference(s)