Ndrg1^str
Spontaneous Allele Detail

Summary

• Symbol: Ndrg1^str
• Name: N-myc downstream regulated gene 1; stretcher
• MGI ID: MGI:5009138
• Synonyms: str
• Gene: Ndrg1 Location: Chr15:66801167-66841489 bp, - strand Genetic Position: Chr15, 29.3 cM, cytoband D3
• Alliance: Ndrg1^str page

Mutation origin

• Strain of Origin: NOD/ShiLtJWehi

Mutation description

• Allele Type: Spontaneous
• Mutation: Intragenic deletion
• Mutation details: More than 5 kilobase pairs of DNA encompassing exons 10-14 have been deleted. Northern blot analysis of kidney RNA reveals the presence in mutant mice of a shorter than normal mRNA, at levels similar to those of the normal transcript in wild-typie littermates; the cDNA sequence confirms that the mutant mRNA has exon 9 spliced in-frame to exon 15. Western blot analysis of protein extracts from sciatic nerve with antibodies to full-length NDRG1 reveals an intense 43-kDa band in wild-type and a very faint band of 32 kDa in mutant sciatic nerve. (J:172770, J:198695)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ndrg1 Mutation: 29 strains or lines available

Notes

The mutant phenotype was observed during production of the NOD.B6-Slc9a1^b/Gem (Chr 4; J:60311) congenic strain. Fine-mapping analysis employing simple sequence length polymorphisms (SSLPs) differentiating NOD, C57BL/6 and DBA/2 (the second parental strain in the mapping cross) demonstrated that the mutation had occurred in a segment of Chr 15 derived from NOD. (J:198695)

References

• Original: J:198695 Chandler D, et al., The stretcher spontaneous neurodegenerative mutation models Charcot-Marie-Tooth disease type 4D [v1; ref status: indexed, http://f1000r.es/8c]. F1000Res. 2013;2
• All: 3 reference(s)