Notch1^tm4(cre)Rko
Targeted Allele Detail

Summary

• Symbol: Notch1^tm4(cre)Rko
• Name: notch 1; targeted mutation 4, Raphael Kopan
• MGI ID: MGI:5009236
• Synonyms: N1::Cre^hi, NIP::Cre^HI, Notch1::cre^Hi
• Gene: Notch1 Location: Chr2:26347914-26393834 bp, - strand Genetic Position: Chr2, 18.91 cM
• Alliance: Notch1^tm4(cre)Rko page

Embryonic lethality in Notch1^tm2Rko/Notch1^tm4(cre)Rko embryos due to the failure of angiogenic remodeling

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171829
• Parent Cell Line: SCC10 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Notch1^tm4(cre)Rko expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Notch1 (MGI:97363)

• Mutation details: The Notch1 intracellular domain (IC) is replaced by the cre recombinase sequence and includes the SV40 3' UTR polyA signal while introducing a single nucleotide variation (SNV) at C5178A to facilitate genotyping. This allele does not have the 6xMyc tag present in the Notch1^tm3(cre)Rko allele, and displays enhanced activity relative to that allele. (J:171829, J:172930)

Generation of the Notch1^tm4(cre)Rko allele

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Notch1 (mouse) Summary of all recombinase alleles driven by Notch1. Alliance mouse Notch1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Notch1 Mutation: 117 strains or lines available

References

• Original: J:171829 Liu Z, et al., Notch1 loss of heterozygosity causes vascular tumors and lethal hemorrhage in mice. J Clin Invest. 2011 Feb 1;121(2):800-8
• All: 7 reference(s)