Etv2^tm2.1Dlim
Targeted Allele Detail

Summary

• Symbol: Etv2^tm2.1Dlim
• Name: ets variant 2; targeted mutation 2.1, Dae-Sik Lim
• MGI ID: MGI:5009241
• Synonyms: Er71^fl
• Gene: Etv2 Location: Chr7:30333041-30335277 bp, - strand Genetic Position: Chr7, 18.92 cM, cytoband A3
• Alliance: Etv2^tm2.1Dlim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:172687
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An FRT-flanked neo cassette with a 5' loxP site was inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 6. Flp-mediated recombination removed the neo cassette and left exons 5 and 6 floxed. (J:172687)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Etv2^tm2.1Dlim/Etv2^tm2.1Dlim	involves: 129S4/SvJaeSor * C57BL/6
cn2	Etv2^tm1Dlim/Etv2^tm2.1Dlim Tg(Mx1-cre)1Cgn/0	involves: 129S4/SvJaeSor * C57BL/6 * CBA

Phenotypes:

Affected Systems	hm1	cn2
show or hide all annotated terms
hematopoietic system		√
impaired myelopoiesis		√
decreased bone marrow cell number		√
abnormal myeloblast morphology/development		√
decreased leukocyte cell number		√
decreased hematopoietic stem cell number		√
spleen hypoplasia		√
abnormal hematopoietic stem cell physiology		√
immune system		√
impaired myelopoiesis		√
decreased leukocyte cell number		√
spleen hypoplasia		√
normal phenotype	√
no abnormal phenotype detected	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	6 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Etv2 Mutation: 14 strains or lines available

References

• Original: J:172687 Lee D, et al., The Er71 is an important regulator of hematopoietic stem cells in adult mice. Stem Cells. 2011 Mar;29(3):539-48
• All: 6 reference(s)