Tg(UBB-EmGFP/RNAi:Sarm1)#Yph
Transgene Detail

Summary

• Symbol: Tg(UBB-EmGFP/RNAi:Sarm1)#Yph
• Name: transgene insertion, Yi-Ping Hsueh
• MGI ID: MGI:5013582
• Transgene: Tg(UBB-EmGFP/RNAi:Sarm1)#Yph Location: unknown
• Alliance: Tg(UBB-EmGFP/RNAi:Sarm1)#Yph page

Impaired dendritic arborization of Tg(UBB-EmGFP/RNAi:Sarm1)#Yph/0 CA1 neurons

Show the 1 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

Transgene Type: Transgenic (Knockdown, Reporter) Mutation: Insertion Tg(UBB-EmGFP/RNAi:Sarm1)#Yph involves 1 genes/genome features (Sarm1) View all Mutation details: The transgene contains a human UBB promoter driving ubiquitous expression of a floxed DsRED*T3/stop cassette, emerald GFP (EmGFP) usptream of two different short hairpins targeting Sarm1, two polyadenylation sequences, and tandem cHS sequences. Cre-mediated recombination removed the DsRED*T3/stop cassette. Three lines were generated with lines 1 and 3 exhibiting higher EmGFP expression compared with line 2. The pound symbol is used when no line is specified and/or lines are pooled. (J:172355)

Generation of the Sarm1 miRNA knockdown transgene Tg(UBB-EmGFP/RNAi:Sarm1)#Yph

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:172355 Chen CY, et al., Sarm1, a negative regulator of innate immunity, interacts with syndecan-2 and regulates neuronal morphology. J Cell Biol. 2011 May 16;193(4):769-84
• All: 3 reference(s)