Pml^{tm1(PML/RARA)Ley}
Targeted Allele Detail

Summary

• Symbol: Pml^{tm1(PML/RARA)Ley}
• Name: promyelocytic leukemia; targeted mutation 1, Timothy J Ley
• MGI ID: MGI:5014059
• Synonyms: mPML-PRflox
• Gene: Pml Location: Chr9:58125359-58157069 bp, - strand Genetic Position: Chr9, 31.63 cM
• Alliance: Pml^{tm1(PML/RARA)Ley} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:172024
• Parent Cell Line: B6/BLU (ES Cell)
• Strain of Origin: C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A cassette, which contains a floxed neo resistance gene with five stop codons in three frames and a fusion of human PML and RARA, was inserted into the 5' untranslated region. The absence of protein expression was confirmed by western blot analysis on spleen cells. (J:172024)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pml Mutation: 91 strains or lines available

References

• Original: J:172024 Welch JS, et al., PML-RARA can increase hematopoietic self-renewal without causing a myeloproliferative disease in mice. J Clin Invest. 2011 Apr 1;121(4):1636-45
• All: 4 reference(s)