Cck^{tm2.1(cre/ERT2)Zjh}
Targeted Allele Detail

Summary

• Symbol: Cck^{tm2.1(cre/ERT2)Zjh}
• Name: cholecystokinin; targeted mutation 2.1, Z Josh Huang
• MGI ID: MGI:5014094
• Synonyms: Cck-CreER
• Gene: Cck Location: Chr9:121318890-121324728 bp, - strand Genetic Position: Chr9, 72.43 cM, cytoband F4
• Alliance: Cck^{tm2.1(cre/ERT2)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Cck^{tm2.1(cre/ERT2)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cck (MGI:88297)

• Mutation details: A targeting vector was designed to insert a CreERT2 fusion gene (Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the cholecystokinin locus (Cck). This construct was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 mice to originate the colony. Mutant mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the neo selection cassette. These Cck-CreER (or Cck-CreERT2) mice were subsequently bred to C57BL/6 inbred mice for two additional generations and then bred together for many more generations (and the FLPe transgene was removed). (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cck (mouse) Summary of all recombinase alleles driven by Cck. Comparative matrix view of recombinase activities Alliance mouse Cck gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cck Mutation: 26 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 9 reference(s)