Cck^{tm1.1(cre)Zjh}
Targeted Allele Detail

Summary

• Symbol: Cck^{tm1.1(cre)Zjh}
• Name: cholecystokinin; targeted mutation 1.1, Z Josh Huang
• MGI ID: MGI:5014096
• Synonyms: Cck-IRES-Cre
• Gene: Cck Location: Chr9:121318890-121324728 bp, - strand Genetic Position: Chr9, 72.43 cM, cytoband F4
• Alliance: Cck^{tm1.1(cre)Zjh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Cck^{tm1.1(cre)Zjh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cck (MGI:88297)

• Mutation details: A targeting vector was designed to insert an internal ribosome entry site (IRES), a cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cholecystokinin locus (Cck). This construct was electroporated into C57BL/6;129 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background) to achieve germline transmission and to remove the neo selection cassette. The resulting pups harboring the cre targeted mutation were selected. These Cck-IRES-Cre mice were subsequently bred together for several generations (and the FLPe transgene was removed). (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cck (mouse) Summary of all recombinase alleles driven by Cck. Comparative matrix view of recombinase activities Alliance mouse Cck gene page

Expression

In Mice Carrying this Mutation:

52018 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cck Mutation: 26 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 111 reference(s)