Dbx1^{tm1.1(cre)Mull}
Targeted Allele Detail

Summary

• Symbol: Dbx1^{tm1.1(cre)Mull}
• Name: developing brain homeobox 1; targeted mutation 1.1, Ulrich Mueller
• MGI ID: MGI:5014205
• Synonyms: Dbx1-IRES-Cre
• Gene: Dbx1 Location: Chr7:49281247-49286583 bp, - strand Genetic Position: Chr7, 31.44 cM
• Alliance: Dbx1^{tm1.1(cre)Mull} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Dbx1^{tm1.1(cre)Mull} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dbx1 (MGI:94867)

• Mutation details: A targeting vector was designed to insert an internal ribosomal entry site, nuclear-localized Cre recombinase gene (NLS-Cre), and polyA signal (IRES-Cre-pA), all followed by an FRT-flanked PGK-Neo cassette into the 3' untranslated region of the developing brain homeobox 1 (Dbx1) locus. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Chimeric mice were bred to albino B6(Cg)-Tyrc-2J/J mice. Next, mutant mice were bred with FLPe-expressing mice on a C57BL/6 congenic background to remove the FRT-flanked PGK-Neo cassette. The resulting Dbx1-IRES-Cre mutant mice were then bred to C57BL/6J inbred mice for many generations (selecting away from the FLPe transgene). (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dbx1 (mouse) Summary of all recombinase alleles driven by Dbx1. Comparative matrix view of recombinase activities Alliance mouse Dbx1 gene page

Expression

In Mice Carrying this Mutation:

10 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dbx1 Mutation: 25 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 7 reference(s)