Wnt3a^{tm1.1(cre)Mull}
Targeted Allele Detail

Summary

• Symbol: Wnt3a^{tm1.1(cre)Mull}
• Name: wingless-type MMTV integration site family, member 3A; targeted mutation 1, Ulrich Mueller
• MGI ID: MGI:5014206
• Synonyms: Wnt3a-IRES-Cre
• Gene: Wnt3a Location: Chr11:59138859-59181578 bp, - strand Genetic Position: Chr11, 37.17 cM
• Alliance: Wnt3a^{tm1.1(cre)Mull} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:151755
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a
• Project Collection: Neuroscience Blueprint cre

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Wnt3a^{tm1.1(cre)Mull} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Wnt3a (MGI:98956)

• Mutation details: A targeting vector was designed to insert an IRES-Cre cassette followed by an FRT-flanked PGK-Neo cassette into the 3' region of the wingless-related MMTV integration site 3A (Wnt3a) locus. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Chimeric mice were bred to albino B6(Cg)-Tyrc-2J/J mice. The resulting Wnt3a-IRES-Cre mutant mice were then bred to C57BL/6J inbred mice for many generations. At some point, Wnt3a-IRES-Cre mutant mice were bred with FLPe-expressing mice on a C57BL/6 congenic background thereby removing the FLPe-expressing transgene. (J:151755)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Wnt3a (mouse) Summary of all recombinase alleles driven by Wnt3a. Comparative matrix view of recombinase activities Alliance mouse Wnt3a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Wnt3a Mutation: 26 strains or lines available

References

• Original: J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009
• All: 9 reference(s)