Slc25a37^tm1.2Kapl
Targeted Allele Detail

Summary

• Symbol: Slc25a37^tm1.2Kapl
• Name: solute carrier family 25, member 37; targeted mutation 1.2, Jerry Kaplan
• MGI ID: MGI:5014804
• Synonyms: Mfrn1^-
• Gene: Slc25a37 Location: Chr14:69479297-69522561 bp, - strand Genetic Position: Chr14, 36.03 cM, cytoband D1
• Alliance: Slc25a37^tm1.2Kapl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173284
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site and FRT flanked neomycin selection cassette were inserted in intron 1 and a second loxP site was inserted in intron 2. The selection cassette was subsequently excised by breeding to mice expressing flp recombinase from the Gt(ROSA)26 Sor promoter. This leaves exon2 flanked by loxP sites. Later, cre expression resulted in excision of exon 2. PCR and Western blot analysis confirmed gene inactivation. (J:173284)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc25a37 Mutation: 9 strains or lines available

References

• Original: J:173284 Troadec MB, et al., Targeted deletion of the mouse Mitoferrin1 gene: from anemia to protoporphyria. Blood. 2011 May 19;117(20):5494-502
• All: 1 reference(s)