Msx1^{tm2.1(cre/ERT2)Bero}
Targeted Allele Detail

Summary

• Symbol: Msx1^{tm2.1(cre/ERT2)Bero}
• Name: msh homeobox 1; targeted mutation 2.1, Benoit Robert
• MGI ID: MGI:5049923
• Synonyms: Msx1-Cre^ERT2, Msx1^CreERT2, Msx1^{tm2(cre/ERT2)Bero}
• Gene: Msx1 Location: Chr5:37977835-37981929 bp, - strand Genetic Position: Chr5, 20.21 cM
• Alliance: Msx1^{tm2.1(cre/ERT2)Bero} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173525
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Msx1^{tm2.1(cre/ERT2)Bero} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Msx1 (MGI:97168)

• Mutation details: A targeting vector with the cre/ERT2 sequence, polyadenylation site, and an Frt-flanked neomycin cassette inserted into the ATG site of Msx1 in a plasmid was generated by homologous recombination in bacteria. The linearized targeting vector was electroporated into ES cells and targeted clones were used to generated chimeras. F1 offspring were crossed to Flp deleter mice to delete the neo marker. (J:173525, J:194129)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Msx1 (mouse) Summary of all recombinase alleles driven by Msx1. Alliance mouse Msx1 gene page

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Msx1 Mutation: 18 strains or lines available

References

• Original: J:173525 Lopes M, et al., Msx genes define a population of mural cell precursors required for head blood vessel maturation. Development. 2011 Jul;138(14):3055-66
• All: 16 reference(s)