Tsx^tm1.2Jtl
Targeted Allele Detail

Summary

• Symbol: Tsx^tm1.2Jtl
• Name: testis specific X-linked gene; targeted mutation 1.2, Jeannie T Lee
• MGI ID: MGI:5050138
• Synonyms: Tsx^KO
• Gene: Tsx Location: ChrX:102458073-102468186 bp, + strand Genetic Position: ChrX, 46.12 cM
• Alliance: Tsx^tm1.2Jtl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177104
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The construct includes an FRT-flanked PGK-neomycin selection cassette upstream of exon 1 followed by a 2.1 kb region encompassing the predicted promoter region upstream of exon 1 and 160 bp of intron 1 flanked by loxP sites. The selection cassette was subsequently excised by FLP recombinase. Cre mediated recombination removed exon 1. Northern blot analysis confirmed loss of all RNA isoforms. (J:177104)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tsx Mutation: 5 strains or lines available

References

• Original: J:177104 Anguera MC, et al., Tsx produces a long noncoding RNA and has general functions in the germline, stem cells, and brain. PLoS Genet. 2011 Sep;7(9):e1002248
• All: 1 reference(s)