Mitf^tm4Arnh
Targeted Allele Detail

Summary

• Symbol: Mitf^tm4Arnh
• Name: melanogenesis associated transcription factor; targeted mutation 4, Heinz Arnheiter
• MGI ID: MGI:5050700
• Synonyms: Mitf^mi-S-73SNeo
• Gene: Mitf Location: Chr6:97784013-97998310 bp, + strand Genetic Position: Chr6, 45.05 cM
• Alliance: Mitf^tm4Arnh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:182722
• Parent Cell Line: LC3 (ES Cell)
• Strain of Origin: (C57BL/6N x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeted mutation modified the splice donor for exon 2B so that it became non functional but did not modify any of the encoded amino acids. The amino acid encoded for codon 73 is Ser. A floxed neomycin selection cassette was inserted as well. This allele forcibly includes exon 2B as it can no longer be alternatively spliced. (J:182722)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mitf Mutation: 74 strains or lines available

References

• Original: J:182722 Debbache J, et al., In vivo role of alternative splicing and serine phosphorylation of the microphthalmia-associated transcription factor. Genetics. 2012 May;191(1):133-44
• All: 1 reference(s)